Fundamental and applied aspects of the hematoencephalic barrier research.Назад
Fundamental and applied aspects of the hematoencephalic barrier research.
Vestn Ross Akad Med Nauk. 2012;(8):66-78.
Chekhonin VP, Baklaushev VP, Iusubalieva GM, Volgina NE, Gurina OI.
Analysis of expression of microRNAs and genes involved in the control of key signaling mechanisms that support or inhibit development of brain tumors of different grades.
Clin Chim Acta. 2014 Mar 20;430:55-62.
Koshkin PA1, Chistiakov DA2, Nikitin AG3, Konovalov AN4, Potapov AA4, Usachev DY4, Pitskhelauri DI4, Kobyakov GL4, Shishkina LV4, Chekhonin VP5
BACKGROUND:
MicroRNAs (miRNAs) are a class of small non-coding RNA molecules involved in the regulation of key biological processes. Different miRNAs with pro-oncogenic and anti-oncogenic properties have been identified in glioblastomas. We decided to analyze expression profiles of 10 mature miRNAs (miR-7-1, miR-10а, miR-17, miR-20а, miR-21, miR-23а, miR-26а, miR-137, and miR-222) in post-surgery glioma specimens of different grades in order to find whether the expression level correlates with tumor grades. We also measured expression of six key genes such as PTEN, p21/CDKN1A, MDR1, ABCG2, BAX, and BCL-2 involved in the regulation of critical glioma signaling pathways to establish the effect of miRNAs on these signaling mechanisms.
METHODS:
Using RT-PCR, we performed expression analysis of 25 tumor fresh samples (grades II-IV).
RESULTS:
We found gradual increase in miR-21 and miR-23a levels in all tumor grades whereas miR-7 and miR-137 were significantly down-regulated depending on the glioma grade. MDR, ABCG2, and p21/CDKN1A levels were significantly up-regulated while expression of PTEN was down-regulated in tumor samples compared to the normal brain tissue.
CONCLUSIONS:
These observations provide new insights into molecular pathogenic mechanisms of glioma progression and suggest about a potential value of miRNAs as a putative diagnostic marker of brain tumors.
